#Qpcr data analysis excel template software
While the automatic settings included in the instrument software are useful and can prevent some wild errors, it is advisable to use common sense and discretion to perform data analysis procedures. Nevertheless, the process of analysing these data is vulnerable to misinterpretation and should be approached with care equal to that exercised during the wet lab processes. Incorporation of the recommendations from the previous articles into a standard operating procedure will go some way towards generating amplification plots that are not just aesthetically pleasing but are the results of efficient qPCR assays and accurately relate to the original nucleic acid sample. However, as we have set out in the first four articles of this series, the translation of an attractive amplification plot into accurate and meaningful data is far from trivial and requires a range of additional considerations. This practical simplicity is complemented by the absence of any requirement for post-assay handling, as well as the development of user-friendly data analysis software that makes data generation and visualisation in the shape of amplification plots remarkably simple. A pivotal attraction of qPCR technology is its apparent lack of complication an assay consisting of the simple procedure of combining oligonucleotides, PCR mastermix buffer and nucleic acid template to produce a qPCR reaction is perceived as undemanding.